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Methadone Confirmation, Urine

Reporting Name

Methadone Confirmation, U

Useful For

Monitoring of methadone treatment for analgesia or drug rehabilitation


Urine measurement of 2-ethylidene-1,5-dimethyl1-3,3-diphenylpyrrolidine is particularly useful for assessing compliance with rehabilitation programs.

Additional Tests

Test ID Reporting Name Available Separately Always Performed
ADULT Adulterants Survey, U No Yes

Testing Algorithm

An evaluation to detect the presence of adulterants will be performed and reported at no additional charge.


See Adulterant Survey Algorithm in Special Instructions.

Method Name

MTDNU: Immunoassay/Gas Chromatography-Mass Spectrometry (GC-MS) Confirmation with Quantitation

ADULT: Spectrophotometry (SP)

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type


Specimen Required

Container/Tube: Plastic, 60-mL urine bottle

Specimen Volume: 20 mL

Collection Instructions:

1. Collect a random urine specimen.

2. No preservative.

Additional Information:

1. For situations where chain-of-custody is required, a Chain-of-Custody Kit (Supply T282) is available. For chain-of-custody information, see COCH / Chain-of-Custody Processing.

2. Additional drug panels and specific requests are available. Call Mayo Medical Laboratories at 800-533-1710 or 507-266-5700.

Specimen Minimum Volume

2.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Urine Refrigerated (preferred) 14 days
  Frozen  14 days
  Ambient  72 hours

Special Instructions

Reference Values


Positives are reported with a quantitative GC-MS result.

Cutoff concentrations:


<300 ng/mL



<100 ng/mL



<100 ng/mL

Day(s) and Time(s) Performed

Monday through Thursday, Sunday

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information


LOINC Code Information

Result ID Reporting Name LOINC Code
21105 Immunoassay Screen 16199-2
56028 EDDP-by GC-MS 50542-0
83129 Methadone-by GC-MS 3774-7
21107 Interpretation 69050-3
21110 Chain of Custody In Process