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Test Code 3400 Flow Helper Cell (CD4) Panel by Flow Cytometry

Methodology

Includes CD45 lymph count and CD3/CD4 T cells.

Performing Laboratory

Our Lady of the Lake Laboratory

Specimen Requirements

ACD and EDTA blood are required.

 

Container/Tube: Lavender-top (EDTA) tube(s) and yellow-top (ACD) tube(s)

Specimen Volume: 3 mL of EDTA whole blood and 7 mL of ACD whole blood

Collection Instructions: Send specimen in original VACUTAINERS®. Do not aliquot.

Specimen Transport Temperature

Refrigerate

Reference Values

T & B SURFACE MARKER

% Helper Cells (CD4)

0-6 months: 50-57%
6-12 months: 49-55%
12 months-2 years: 42-48%
2-3 years: 33-44%
3-15 years: 27-57%
15-150 years: 34-54%

% CD45 Lymph Count, Flow

Males

0-1 day: 26-36%
1 day-6 years: 35-62%

6-12 years: 22-59%
12-150 years: 21-47%
Females

0-1 day: 26-36%

1 day-6 years: 29-62%
6-12 years: 26-58%
12-150 years: 21-47%

% T Cells (CD3)

0-15 years: 55-82%

15-150 years: 63-84%

ABSOLUTE COUNTS

Helper Cells (CD4)

0-6 months: 2,780-3,908/mm3

6-12 months: 2,630-3,499/mm3
12 months-2 years: 1,919-2,472/mm3
2-3 years: 1,216-2,009/mm3
3-15 years: 562-2,692/mm3
15-150 years: 430-1,276/mm3

CD45 Lymph Count, Flow

Males

0-8 days: 1,000-9,000/mm3
8 days-1 month: 1,000-11,000/mm3
1 month-2 years: 1,400-10,000/mm3
2-6 years: 1,700-4,200/mm3
6-12 years: 1,500-3,400/mm3
12-150 years: 1,300-2,900/mm3

Females

0-8 days: 1,000-9,000/mm3
8 days-1 month: 1,000-11,000/mm3
1 month-2 years: 1,400-10,000/mm3
2-6 years: 1,600-4,200/mm3
6-12 years: 1,400-3,400/mm3
12-150 years: 1,300-2,900/mm3

T Cells (CD3)

0-6 months: 3,505-5,009/mm3
6-12 months: 3,409-4,575/mm3
12 months-2 years: 2,766-3,508/mm3
2-3 years: 1,923-3,141/mm3
3-15 years: 1,072-3,890/mm3
15-150 years: 630-3,360/mm3
WBC FLOW
0-1 day: 13.0-38.0 mm3 x 103
1-7 days: 5.0-21.0 mm3 x 103
7-14 days: 5.0-20.0 mm3 x 103
14 days-1 month: 5.0-19.5 mm3 x 103
1 month-1 year: 6.0-17.5 mm3 x 103
1-2 years: 6.0-17.0 mm3 x 103
2-4 years: 5.5-15.5 mm3 x 103
4-6 years: 5.0-14.5 mm3 x 103
6-12 years: 4.5-13.0 mm3 x 103
12-150 years: 4.5-10.8 mm3 x 103

Day(s) Test Set Up

Monday through Friday

Test Classification and CPT Coding

88184-Flow cytometry, first marker

88185 x 2-Flow cytometry, each additional marker